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Mcf10a-ras

Web31 jul. 2016 · Stable MCF10A-Ras cells were established by transfection of LXSN-K-Ras vector into MCF10A cells; MCF7 wt and drug-resistant sublines were from Dr. A.M. Parissenti, Laurentian University, Sudbury, ON, Canada ( 9 ). Web22 jun. 2024 · Methods: The purpose of the current studies was to determine the effects of detachment on glucose and glutamine metabolism in human breast epithelial MCF10A …

Using the MCF10A/MCF10CA1a Breast Cancer Progression Cell …

Web26 jan. 2009 · In this study, we show that transformation of MCF10A cells by Harvey-Ras (Ha-Ras) induces CCAAT/enhance binding protein β (C/EBPβ) and activates the … Web15 feb. 2010 · To gain insight into the role of genomic alterations in breast cancer progression, we conducted a comprehensive genetic characterization of a series of four cell lines derived from MCF10A. MCF10A is an immortalized mammary epithelial cell line (MEC); MCF10AT is a premalignant cell line generated from MCF10A by transformation … discov3ry complete https://opti-man.com

ErbB2-enhanced invasiveness of H-Ras MCF10A breast cells …

Web28 dec. 2024 · MCF10A- ras cells were transfected with the pCMV6-Neo or pCMV6-GOT1 (NM_002079) expressing plasmid (Origene Technologies, Rockville, MD) using lipofectamine 2000 and Opti-MEM Reduced Serum Medium (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. Web12 mrt. 2024 · We report the synthesis and preliminary characterization of IODVA1, a potent small molecule that is active in xenograft mouse models of Ras-driven lung and breast cancers. In an effort to inhibit oncogenic Ras signaling, we combined in silico screening with inhibition of proliferation and colony formation of Ras-driven cells. NSC124205 fulfilled … Web13 mei 2015 · MCF10A is a spontaneously immortalized, non-malignant breast cell line obtained from a patient with benign fibrocystic disease and is the founder cell line of a … discouted day timer refills

Mitochondrial fission links ECM mechanotransduction to ... - Nature

Category:KRAS signaling enriched triple negative breast cancer is associated ...

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Mcf10a-ras

H-Ras induces Nrf2-Pin1 interaction: Implications for breast cancer ...

Web27 okt. 2024 · 1. Analysis of TGF-β-induced SMAD2 phosphorylation using Western blotting. NOTE: Premalignant human breast MCF10A-Ras cells were used as an example to investigate TGF-β signaling responses 33.In principle, the methods described below are also applicable to other TGF-β-responsive cell lines. Web11 apr. 2024 · We developed an in vivo assay based on the immortalized nontumorigenic breast cell line MCF10A and its Ras-transformed …

Mcf10a-ras

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WebThe MCF10 human breast epithelial model system consists of mortal MCF10M and MCF10MS (mortal cells grown in serum-free and serum-containing media, respectively), immortalized but otherwise normal MCF10F and MCF10A lines (free-floating versus growth as attached cells), transformed MCF10AneoT cells transfected with T24 Ha-ras, and … WebRas and c-Myc induce epithelial to mesenchymal transition in MCF10A mammary epithelial cells. (A) Twist expression was examined by immunoblotting in MCF10A-Ras and …

Web16 mrt. 2009 · While few established breast cancer cell lines have Ras mutations, a majority have Ras overexpression via amplification of the gene [31–33]. More recently, activating mutations in H-ras, K-ras, or N-ras were observed in 8 out of 40 human breast cancer cell lines, with another 4 lines having activating mutations in B-Raf, which is downstream in … WebActivation of the Ras pathway is also frequently found in breast ... into the mechanisms behind collective migration by studying cell migration in a …

Web28 mrt. 2011 · The MCF10A-Myc cells, in contrast, were less responsive to this external cue and maintained a high level of deregulated Myc protein expression (Figure 1a, compare … Web(C) MCF10A-ras cells were transfected with siH-RAS, then the cells were treated with 200 μM Eu. The protein expression of c-Myc, PGC-1β and ERRα were measured by western blot. β-actin was used ...

Web13 mei 2008 · The ability of MCF10A-Ras cells to invade a collagen matrix was assessed ( 15 ), because this process may more closely recapitulate the in vivo invasive phenotype. The refractile cells identified distal to the cellular margin after 5 days were used as a measure of cellular invasion of collagen.

Web1α,25-dihydroxyvitamin D reduction of MCF10A-ras cell viability in extracellular matrix detached conditions is dependent on regulation of pyruvate carboxylase 1α,25 … discou ted amp card for pa speakerWeb1 jul. 2024 · For expression in the MCF-10A cells, both NeuT and NeuTY1143F mutants were flag tagged at the C-terminus and subcloned into the same retroviral vector. Plasmids used are as follows. Gene expression in NR6 cells and MCF10A was done using the retroviral pMSCV PIG vector (gift of David Bartel, addgene #21654). discova office in hanoiWeb23 feb. 2015 · (a) Immunoprecipitation of GTP-bound KRAS from EV-transduced MCF10A cells or MCF10A clones expressing the indicated KRAS mutants at low levels following stimulation with EGF (20 ng/ml) for 10... discova singapore city sights