Web31 jul. 2016 · Stable MCF10A-Ras cells were established by transfection of LXSN-K-Ras vector into MCF10A cells; MCF7 wt and drug-resistant sublines were from Dr. A.M. Parissenti, Laurentian University, Sudbury, ON, Canada ( 9 ). Web22 jun. 2024 · Methods: The purpose of the current studies was to determine the effects of detachment on glucose and glutamine metabolism in human breast epithelial MCF10A …
Using the MCF10A/MCF10CA1a Breast Cancer Progression Cell …
Web26 jan. 2009 · In this study, we show that transformation of MCF10A cells by Harvey-Ras (Ha-Ras) induces CCAAT/enhance binding protein β (C/EBPβ) and activates the … Web15 feb. 2010 · To gain insight into the role of genomic alterations in breast cancer progression, we conducted a comprehensive genetic characterization of a series of four cell lines derived from MCF10A. MCF10A is an immortalized mammary epithelial cell line (MEC); MCF10AT is a premalignant cell line generated from MCF10A by transformation … discov3ry complete
ErbB2-enhanced invasiveness of H-Ras MCF10A breast cells …
Web28 dec. 2024 · MCF10A- ras cells were transfected with the pCMV6-Neo or pCMV6-GOT1 (NM_002079) expressing plasmid (Origene Technologies, Rockville, MD) using lipofectamine 2000 and Opti-MEM Reduced Serum Medium (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. Web12 mrt. 2024 · We report the synthesis and preliminary characterization of IODVA1, a potent small molecule that is active in xenograft mouse models of Ras-driven lung and breast cancers. In an effort to inhibit oncogenic Ras signaling, we combined in silico screening with inhibition of proliferation and colony formation of Ras-driven cells. NSC124205 fulfilled … Web13 mei 2015 · MCF10A is a spontaneously immortalized, non-malignant breast cell line obtained from a patient with benign fibrocystic disease and is the founder cell line of a … discouted day timer refills