Phospho erk antibody cst
WebPhospho-ERK1/2 (Thr202, Tyr204) Antibody (14-9109-82) in IHC (P) Immunohistochemistry of formalin-fixed paraffin embedded human breast cancer tissue stained with 5 µg/mL of … WebThe Phospho-ERK (Thr202/Tyr204) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. The first antibody is selected for its specific binding to the phosphorylated motif on the …
Phospho erk antibody cst
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WebApr 11, 2024 · Dox removal results in loss of Kras G12D expression and ERK activity in iKras PDAC cells. Cells were grown in the presence or absence of Dox for the indicated amounts of time, before being subjected to lysis and immunoblotting (IB) with the indicated antibodies. ... Cell Signaling: 4695S: Anti-Phospho-p44/42 MAPK (Thr202/Tyr204) (p … WebWestern blot band for Erk and phopho (p)-Erk I want to know Erk activity in my animal model - sepsis. I isolated it from liver, homogenized it, separated it by SDS-PAGE, and detected it, but...
WebImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PERK (phospho T982) antibody (ab192591) Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue labeling PERK (phospho T982) with ab192591 at a 1/50 dilution. The image on the right shows the same antibody preincubated with blocking … WebUpon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization. Flow Cytometry Panel Builder
WebMay 14, 2024 · After initiating T-cell signaling with the dimerizer, we pulsed the cell conjugates with light after 5 min for 3 min before returning them to the signaling-competent state. ... The resuspended pellet was then incubated with the phospho-ERK1/2 (Thr 202 /Tyr 204) antibody directly conjugated with Alexa Fluor 488 for 45 min at room temperature ... WebJan 18, 2024 · Phosphorylation of ERK is increased by CCN2. ( A) LECs were stimulated with 100 ng/mL CCN2 for 0, 5, 15 or 30 min, and phosphorylated ERK, total ERK and HSP90 were detected by Western...
Web(9527) Phospho-cdc25C (Thr48) Antibody - Cell Signaling Technology - CiteAb 14 citations – A rabbit polyclonal antibody, raised against M-phase inducer phosphatase 3 (Human), supplied by Cell Signaling Technology.
WebPhospho-PERK (Thr982) Antibody (PA5-40294) in WB. Oncology reports 2024 - Figure 3. DHA activates the UPRs in PLC cells in vitro . HepG2 and PLC/PRF/5 cells were treated with 40 and 25 uM DHA, respectively, for 1, 6, 12 or 24 h. (A and C) The protein expression levels of UPR-associated molecules were assessed by western blotting. philippians 3 word searchWebCell Signaling Technology's Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Precipitation, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blot. trulli of albeWebThe Phospho-Specific Mouse Anti-EGF Receptor pSer1047 ( VMA00746) and anti-total Rabbit Anti-EGF Receptor were incubated together. hFAB Rhodamine Anti-Tubulin was added with the fluorescent secondary antibodies. The fluorescent images were captured using the ChemiDoc MP Imaging System. 5 Phosphatase treatment philippians 4 11 12 commentaryWebPhospho Erk Erk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, … trulli of alberobello 1WebAug 15, 2003 · Activation of AKT and ERK-1/2 was assessed using 1:1000 dilutions of antiphospho-specific antibodies (Cell Signaling Technology). Horseradish peroxidase–conjugated secondary antibodies were used to develop the membrane. The protein bands were detected with Supersignal chemiluminescence substrate (Pierce … philippians 4:10 strength in sufferingWebThis antibody is predicted to react with Xenopus and zebrafish based on 100% sequence homology. 36-8800 is specific for the ~42k - 44k ERK/MAPK phosphorylated at Thr202 and Tyr204. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. philippians 4:10-20 commentaryWebNov 20, 2024 · Phosphorus is a mineral the body needs to build strong bones and teeth. It is also important for nerve signaling and muscle contraction. This test is ordered to see how … trull ofsted